human neuroblastoma cell line be2c  (ATCC)

Journal: iScience

Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis

doi: 10.1016/j.isci.2026.115144

Figure Lengend Snippet: Macrophages cultured with conditioned media from LMO1-overexpressing BE2C cells accelerate tumor-cell migration in vivo (A) Schematic diagram of experimental setup. U937 human monocytes were first differentiated to M0 macrophages with phorbol 12-myristate 13-acetate (PMA) and then cultured in conditioned media (CM) derived from control (“Ctl”) or LMO1-overexpressing (“LMO1”) BE2C cells. Ctl-CM-treated and LMO1-CM-treated U937 cells were labeled with ViaFluor 405 (green-fluorescent) dye. Each group of 405-labeled U937 cells was then combined with mCherry-labeled BE2C cells and co-injected into 2-day-old zebrafish through the perivitelline space (PVS, see diagram for the location of injection). (B–I) Ventral-view (B-E) or lateral-view (F-I) fluorescent images of transplanted zebrafish larvae at 1 day post-injection (dpi, left panels) or 3–4 dpi (right panels) (scale bars, 100 μm). Blue double arrowheads point to the site of injection in panels B-E. Black arrows point to U937 macrophages. Red arrows point to BE2C NB cancer cells. (J) Quantification of transplanted fish with NB cell migration ( n = 55). Migration of NB cells in transplanted recipients was defined as at least one or more mCherry-positive tumor cells located in the anterior regions of the fish, distant from the site of injection (outside of PVS and yolk). ∗∗∗∗ p ≤ 0.0001.

Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).

Techniques: Cell Culture, Migration, In Vivo, Derivative Assay, Control, Labeling, Injection

Journal: iScience

Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis

doi: 10.1016/j.isci.2026.115144

Figure Lengend Snippet: Increased levels of metastasis-promoting cytokines are detected in the CM derived from LMO1-overexpressing BE2C cells (A) Cytokine arrays were probed using CM from Ctl BE2C cells (left) and LMO1-overexpressing BE2C cells (right) ( n = 2). Metastasis-promoting cytokines are labeled as follows: red boxes - Angiogenin; orange boxes - angiopoietin-2; green boxes - CD14; light-blue boxes - EGF; dark-blue boxes - Endoglin/CD105; purple boxes - GDF-15; pink boxes - HGF; gray boxes - ICAM-1/CD54; black boxes - CCL2/MCP-1; neon-green boxes - CXCL12/SDF-1 alpha; yellow boxes - VEGFA. (B) Quantitation of relative cytokine levels based on the normalized signal intensity on two independent cytokine array blots ( n = 2). (C) Relative expression of genes (determined by qRT-PCR) encoding the indicated cytokines in Ctl and LMO1-overexpressing BE2C cells ( n = 4). Data in (B) and (C) are presented as mean ± SD. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. ns; not significant.

Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).

Techniques: Derivative Assay, Labeling, Quantitation Assay, Expressing, Quantitative RT-PCR